RIDASCREEN® Acetylgestagene is a competitive enzyme immunoassay for the quantitative analysis of medroxprogesterone acetate (MPA) and other acetylgestagenes in bovine perirenal fat.
All reagents required for the enzyme immunoassay - including standards - are contained in the test kit.
For in vitro use only.
General Information:
Medroxyprogesterone acetate (MPA), 17α-acetoxyprogesterone, megestrol acetate und chlormadinone acetate belong to the group of acetylgestagenes. Acetylgestagenes are known to increase the efficiency of animal production and have been used in illegal cocktails for calf fattening in the past. However these substances are not permitted as resources for animal fattening. Some studies have shown that the peak-concentrations of acetylgestageneresidues can be observed in fat tissue. MPA, for instance, can be detected in plasma only a few days past application but it cannot be detected with accuracy in urine. After the application of MPA to calves high concentrations of nonmetabolized MPA can be detected in fat, even after 19 days.
Test Format: Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Incubation Time: 2 hrs 30 min
Detection Limit: 0.3-0.6 ppb
Cross-reactivity:
- Medroxyprogesterone acetate: 100%
- 17α-Acetoxyprogesterone: 126%
- Megestrol acetate: 50%
- Chlormadinone acetate: 50%
- Melengestrol acetate: 0.6%
- Cross reaction of all other substances being checked in the assay: ≤ 0.002%