RIDASCREEN® Melengestrolacetat is a competitive enzyme immunoassay for the quantitative analysis of melengestrol acetate in bovine perirenal fat and muscle meat.

All reagents required for the enzyme immunoassay - including standards - are contained in the test kit.

For in vitro use only.

General Information:

Synthetic gestagens can be used for estrus inhibition or synchronisation after break off and improvement of fertility. In addition one of these substances, melengestrol acetate (MLGA), is a licensed growth promoting feed additive for heifers in the USA and Canada. The admitted dose is 0.5 mg per day and head, given as a feed premix. Its mode of anabolic action is unclear, but it stimulates the ovarian synthesis of the endogenous anabolic steroid estradiol and that may have androgenic side effects. MLGA belongs to the most active synthetic gestagens. Its oral bioactivity is about 10 or 100 times higher than the activities of the gestagens chlormadinone acetate (CMA) or medroxyprogesterone acetate (MPA). If given parenterally the hormonal activity of MLGA is still 125 times higher than the one of progesterone. Due to its strongly lipophilic properties, MLGA is accumulated in fat 200-fold higher than in blood plasma.

In the European Union the use of sexual hormones for growth promoting purposes is generally forbidden since 1988, and also the import of meat from hormone treated cattle into the EU is prohibited.

Test Format: Microtiter plate with 96 wells (12 strips with 8 removable wells each)

Incubation Time: 2 hrs 30 min

Detection Limit:

• bovine perirenal fat: 0.3 ppb
• muscle: 0.075 ppb

Cross-reactivity:

• Melengestrol acetate (MLGA): 100%
• Megestrol acetate (MGA): 10%
• Medroxyprogesterone acetate (MPA): 6.6%
• 6α-Methyl-17α-OH-Progesterone acetate: 5.2%
• Chlormadinone acetate (CMA): 4.8%
• 17α-Acetoxyprogesterone: 3.9%
• 16α-Methylprogesterone: 0.0035%
• all other tested substances: < 0.003%