With: L - Glutamine

Without: Phenol red and Sodium bicarbonate

AME’s Medium was specially formulated to maintain rabbit retina in vitro. Rabbit retina is a good model for studying relationships between function and metabolism in organized mammalian central nervous tissue. Rabbit retina offers several advantages as it is easily accessible and strong enough to remain intact during manipulations. AME’s Medium is formulated to closely resemble the composition of the cerebrospinal fluid that bathes the retina in vivo. Morphologic, metabolic, and electrophysiologic measurements obtained on the in vitro retinas showed that they remained in a nearly physiological state for at least 8 hours, and even after 2 days in vitro they still exhibited a high level of metabolic activity and electrical responsiveness to light. AME’s medium therefore remains the medium of choice for maintaining central nervous tissue in vitro.

AT121 is AME’s Medium with L-glutamine. Its does not contain Sodium bicarbonate and Phenol red. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.