With: L-Glutamine, Phenol red, 0.2% Glucose and 0.165 moles per litre MOPS buffer

Without: Sodium bicarbonate

Roswell Park Memorial Institute (RPMI) media are a series of media developed by Moore et al for the culture of human normal and neoplastic cells in vitro. RPMI 1640 is the most commonly used medium in the series. A modification of McCoy’s 5A medium, the medium was specifically designed to support the growth of human lymphoblastoid cells in suspension culture. Presently the medium is extensively used for a wide range of anchorage dependant cell lines. The medium needs to be supplemented with 5-20% fetal bovine serum. The medium is also known to support growth of cells in the absence of serum.

AT180 is RPMI 1640 with L-glutamine, 2gms per litre glucose, 0.165M per litre MOPS buffer. It does not contain sodium bicarbonate. MOPS, is a zwitterionic buffer does not antagonize antifungal agents at final concentration of 0.165mol/L for pH 7.0. Therefore, this medium is used as a diluent for antifungal agents that are water-soluble as well as water-insoluble. For waterinsoluble antifungal agents, that cannot be prepared as stock solutions in water, such as amphotericin B, anidulafungin, itraconazole, ketoconazole, posaconazole and voriconazole, a dilution series of the agent should be prepared first at 100 times final strength in an appropriate solvent. Each of these non-aqueous solutions should then be diluted tenfold in RPMI-1640 broth.