With: Trace elements and Sodium bicarbonate

Without: L-Glutamine and HEPES buffer

LoSera™ media are based on the classical formulations supplemented with insulin, transferrin and other advanced nutrients. The additional nutrients help in reducing the percentage of serum required to grow mostof the common cell lines. The percentage of serum reduction may vary with type of cell line used. For non-fastidious cell lines serum can be reduced from 10% to as low as 1%. For fastidious cell lines serum usage can be reduced from 10% to 2.5%. LoSera™ medium can be used without prior adaptation and sub cultured using normal procedures. Reduced serum supplementation improves the reproducibility of experimental results by decreasing the variability caused due to undefined serum constituents. It also facilitates down regulation process in bioassays and in purification process of culture products.

Dulbecco’s Modified Eagle Medium / Nutrient Mixture F-12 Ham (DMEM / F12 1:1 Mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells.

RSL005 is LoSera™ DMEM Nutrient Mixture F-12 Ham (1:1) with Trace elements and Sodium bicarbonate, It does not contain L-Glutamine and HEPES buffer. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.

1X Liquid Cell Culture Medium requiring reduced serum supplementation