With: 15 mM HEPES buffer and Sodium bicarbonate
Without: L-Glutamine and Trace elements
Dulbecco’s Modified Eagle Medium / Nutrient Mixture F-12 Ham (DMEM / F12 1:1 Mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells.
AL140 is DMEM Nutrient Mixture F-12 Ham (1:1) with 15mM HEPES buffer and Sodium bicarbonate. It does not contain Trace elements and L-Glutamine. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
1X Liquid Cell Culture Medium