With: L-Alanyl-L-Glutamine, Sodium bicarbonate, 15mM HEPES buffer and Trace elements

HiGlutaXL™ medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build -up of ammonia. L-alanyl-L-glutamine incorporates L-alanine thatprotects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The thermostable nature of L-alanyl-L-glutamine obviates the need of addition of L-glutamine at the time of use and allows liquid media to be stored at 4°C for longer periods.

Dulbecco’s Modified Eagle Medium / Nutrient Mixture F-12 Ham (DMEM / F12 1:1 Mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells.

AL139G is HiGlutaXL™ DMEM Nutrient Mixture F-12 Ham (1:1) with L-Alanyl-L-Glutamine, Sodium bicarbonate, 15mM HEPES buffer and Trace elements. HEPES a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium. It does not contain HEPES buffer and Trace elements. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.

1X Liquid Cell Culture Medium