ONPtest is designed for the ß-galactosidase detection. ß-galactosidase is an intracellular enzyme atalyzing the cleavage of lactose. Lactose fermentation into monosaccharides galactose and glucose is dependent on another enzyme- permease, which is necessary for the penetration of lactose into the bacterial cell. In the absence of permease a ß-galactosidase-positive organism will not ferment lactose. In such cases it is advantageous to use ONPtest. Enzyme ß-galactosidase hydrolyses the colourless substrate of O-nitrophenyl-ß-galactopyranoside; the positive reaction is detected by yellow colouring of free O-nitrophenol.

• The strip is dipped into a defined bacterial suspension and incubated.
• Reaction can be evaluated within 4, 24 or 48 hours according to the strain type.

MIKROLATEST® detection strips are plastic strips with a porous zone made of filter paper containing reagent substrate for detection of bacterial enzyme or metabolite. They are a modern alternative to diagnostic discs, they are more user friendly and economical. According to their types, the bacterial culture tested is either rubbed into the reagent zone or the strip is inserted into a tube containing an examined bacterial suspension and cultured.

The detection strips are used either together with MIKROLATEST® identification kits for the additional testing or separately. In some cases, additional reagents should be used in order to improve reaction sensitivity. One strip package is sufficient for 50 examinations. Features of

MIKROLATEST® detection strips:

• user-friendly – easy manipulation without any other aids
• reliable quality – standard and reproducible results
• fast detection – results available within 1 minute, 1-4 minutes or not later than within 48 hours, according to the test and the microorganism type

MIKROLATEST® detection strips include:

• plastic tube containing 50 pcs of strips
• instructions for use
• storage - detection strips should be stored in a refrigerator at the temperature of +2 up to +8 °C