GENERAL INFORMATION

The Autof MS 1000 is a Matrix Assisted Laser Desorption Ionisation–Time of Flight (MALDI-TOF) mass spectrometry microbial identification system. Autof MS 1000 provides automated, high-speed and high-confidence identification and taxonomical laser-induced classification of bacteria, yeasts, fungi, and filamentous fungi based on proteomic fingerprinting. Numerous studies have demonstrated the higher accuracy, faster time-to-result, and lower cost provided by MALDI-TOF technology when compared to classical methods.

SAMPLE WORKFLOW

PRINCIPLE OF THE METHOD

MALDI’s purpose is to fire a laser on to the co-crystallisation film formed by the target plate samples and matrix. The matrix absorbs energy from the laser and transmits it to the sample, heating up and vaporising a small quantity of matrix and sample. The process of ionisation is when the target molecules receive or donate protons, making them charged ions. The TOF principle is to accelerate the ions under a uniform electric field, meaning the differences in acceleration are based on the different masses of the ions (they all carry the same charge), and then to plot them on a graph with mass to charge ratio (m/z) on the x-axis and relative abundance or intensity on the y axis.

FEATURES

• Library of > 5 300 species; > 18 375 strains, which is renewed and enriched regularly and free of charge
• Faster time-to-result when compared to conventional methods and PCR
• Target plate has area for 96 samples, which would take approximately 17 minutes to analyse
• Cleaning free ion source, eliminating the need for manual cleaning or cleaning with the laser beam
• One piece of software for both target analysis and scoring
• Cloud based database which can be accessed from anywhere and updated for free

SPECIFICATIONS