Tick-Borne diseases comprise a group of infections transmitted to humans by the bite of ticks infected with bacteria, viruses, or parasites. Tick-Borne diseases affecting humans include Lyme disease, relapsing fever, babesiosis, anaplasmosis and Q fever among others.
Lyme disease (or Lyme borreliosis) is the most common Tick-Borne disease worldwide. It is caused by the spirochaete Borrelia burgdorferi and it is transmitted to humans through the bite of infected blacklegged ticks of the genus Ixodes.
Borrelia miyamotoi and B. hermsii are species of spiral-shaped bacteria that is closely related to the bacteria that cause tick-borne relapsing fever (TBRF).
Anaplasmosis is caused by the bacterium Anaplasma phagocytophilum and is transmitted to humans through ticks of the genus Ixodes.
Q fever is caused by the bacteria Coxiella burnetii. Cattle, sheep, and goats are the primary reservoirs of Coxiella burnetii and transmission to humans occurs primarily through inhalation of aerosols from contaminated soil or animal waste, though it can be transmitted through tick bites.
Since most Tick-Borne diseases show similar symptoms, differential diagnosis can be difficult. PCR assays have been shown to be the most specific diagnostic tool for their detection and type differentiation.
VIASURE Borrelia, Anaplasma & Coxiella Real Time PCR Detection Kit is designed for the specific identification and differentiation of Borrelia burgdorferi sensu lato, Borrelia miyamotoi and/or Borrelia hermsii, Anaplasma phagocitophilum and/or Coxiella burnetii in clinical samples from patients with signs and symptoms of Tick-Borne diseases.
VIASURE Borrelia, Anaplasma & Coxiella Real Time PCR Detection Kit contains in each well all the components necessary for real time PCR assay (specific primers/probes, dNTPS, buffer, polymerase) in a stabilized format, as well as an internal control to discard the inhibition of the polymerase activity.
Materials and reagents provided in the kit:
- Borrelia, Anaplasma & Coxiella 8-well strips – 12 x 8-well strip
- Rehydration Buffer – 1 x 1.8 m
- Positive Control (Borrelia, Anaplasma & Coxiella) – 1 via
- Negative control – 1 via
- Water RNAse/DNAse free – 1 x 1 m
- Tear-off 8-cap strips – 12 x 8-cap strip