The genus Bordetella is comprised of 8 species, 4 of which are known to infect humans; B. pertussis, B. parapertussis, B. holmesii, and B. bronchiseptica. The most important cause of whooping cough (pertussis) is Bordetella pertussis.

Pertussis is a very contagious disease that spreads from person to person usually by coughing or sneezing or when spending a lot of time near one another where you share breathing space. The clinical course of the illness is divided into three stages which include the following clinical features: catarrhal (coryza, low-grade fever, mild and occasional cough), paroxysmal (paroxysms of numerous and rapid coughs, cyanosis, vomiting, and exhaustion), and convalescent (gradual recovery and less persistent paroxysmal coughs).

Despite vaccination pertussis remains endemic in most areas of the world. Reliable diagnosis is required to start appropriate treatment and prophylaxis of contacts if needed, particularly non vaccinated infants in whom pertussis might present as a life-threatening disease. Nucleic acid amplification tests, including PCR and more recently real-time PCR, overcome some of the limitations of culture and serological methods for the diagnosis of Bordetella infections. Most of the PCR tests are based on detection of insertion sequences (IS) present in multiple copies per genome, increasing the sensitivity of PCR tests.

VIASURE Bordetella Real-Time PCR Detection Kit is designed for specific detection and differentiation of Bordetella pertussis, Bordetella parapertussis and/or Bordetella holmesii in respiratory samples from patients with signs and symptoms of respiratory infection.

VIASURE Bordetella Real-Time PCR Detection Kit contains in each well all the components necessary for real time PCR assay (specific primers/probes, dNTPS, buffer, polymerase) in a stabilized format, as well as an internal control to discard the inhibition of the polymerase activity.

Materials and reagents provided in the kit:

• Bordetella 8-well strips – 12 x 8-well strip
• Rehydration Buffer – 1 x 1.8 m
  
• Positive Control (Bordetella ) – 1 via
  
• Negative control – 1 via
  
• Water RNAse/DNAse free – 1 x 1 m
  
• Tear-off 8-cap strips – 12 x 8-cap strip

Storage: from 2 ºC to 40ºC (room temperature)

Expiration date: 24 months

CE-IVD certified