RIDASCREEN®FAST Hazelnut is a sandwich enzyme immunoassay for the quantitative analysis of hazelnut (e.g. traces of hazelnut) in food such as cereals, bakery products, ice cream and chocolate.
The ELISA has been validated for dark chocolate by the German BVL (§64 method LFGB - 15633-2 method).
All reagents required for the enzyme immunoassay - including standards - are contained in the test kit.
For in vitro use only.
General Information:
The allergen can be present as an ingredient or as a contamination in raw and cooked products. According to the Regulation (EU) No. 1169/2011, hazelnut and products thereof must be declared on food labels. Similar regulations exist e.g. in the USA, Canada, Australia and New Zealand.
Test Format: Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Standard Range: 0 ppm (zero standard), 2.5 ppm, 5 ppm, 10 ppm, 20 ppm hazelnut
Sample Preparation: homogenization, extraction and centrifugation
Incubation Time: 30 min
Detection Limit:
- Limit of Detection: 1.5 mg/kg (ppm) or 0.00015% hazelnut in the sample
- Limit of Quantification: 2.5 mg/kg (ppm)
Cross-reactivity: sun flower seeds: ≤ 0.07%; linseeds (ground), almonds: no. The detection depends on the degree of roasting.
YouTube - Allergen Analysis Test Procedure for the Sandwich ELISA RIDASCREEN®FAST
