RIDASCREEN®FAST Lupine is a sandwich enzyme immunoassay for the quantitative analysis of lupine proteins in food like beverages (juice, wine, beer), sausages and meat products, baking-mixtures for cakes or bread, bakery goods, pasta, chocolate, milk products and ice cream.
All reagents required for the enzyme immunoassay - including standards - are contained in the test kit.
For in vitro use only.
General Information:
The macroscopic granular sweet lupines contain 35-42% proteins in the seeds. Lupins are generally used as a natural protein source as an alternative to soy- and animal products in human and animal food. They are able to bind water in food and therefore can be used as a replacement of egg in bread and bakery products. This increases the freshness and stability of those products. Beside this lupine flour is used instead of wheat, rye or barley flour in the case of dietetic food. In France it is allowed to mix up to 10% lupin flour to other flours without further declaration.
Due to the fact that lupines can cause severe allergic reactions they should be declared in food products. Additionally in the case of peanut allergic persons a cross allergization between peanut and lupines can occur. The European legislative 2007/68//EC from November, 27. 2007, Annex III a has lupines on the list as an allergen which has to be declared on the label.
The RIDASCREEN®FAST Lupine test measures the contamination of food by lupine proteins or small amounts of lupine flour as an ingredient in raw and cooked products.
Test Format: Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Standard Range: 0 ppm (zero standard), 0.05 ppm, 0.1 ppm, 0.2 ppm, 0.4 ppm lupine
Sample Preparation: homogenization, extraction and centrifugation
Incubation Time: 30 min
Detection Limit:
- Limit of detection: 0.6 mg/kg (ppm) lupine protein
- Limit of quantification: 1 mg/kg (ppm) lupine protein
Cross-reactivity: The antibody specifically detects sweet lupine proteins, including the γ-conglutin as well as all european species of sweet lupines (Lupinus albus, luteus andangustifolius) relevant in the food and feed industry.
Cross-reactivity to chickpeas: 0.31%
YouTube - Allergen Analysis Test Procedure for the Sandwich ELISA RIDASCREEN®FAST